Captain Courtney Lawes will not be playing in England’s match with Argentina at Twickenham this Sunday.
Lawes continues to feel symptoms after suffering a head trauma in September. He will not be joining the squad this Wednesday.
Owen Farrell is now back from a concussion he sustained against Exeter Chiefs in October.
The 31 year-old will go on to the final stages of the process.
Lewis Ludlam also has been out with injury. Meanwhile, Jonny May, winger, has made quick recovery from an elbow issue to rejoin the 36-man squad.
Friday will see the announcement of matchday 23.
The first game in the Autumn Nations Series is England’s Test match against Argentina. They will also be playing New Zealand, South Africa, and Japan.
Argentina squad: England full
Forwards: Alex Coles, Northampton Saints, Luke Cowan-Dickie(Exeter Chiefs), Tom Curry/Sale Sharks), Ellis Genge/Bristol Bears), Joe Heyes/Leicester Tigers), Jonny Hil (Sale Sharks), Maro Itoje (Saracens), George McGuigan/Newcastle Falcons), Tom Pearson/London Irish), Val Rapava Ruskin/Gloucester Rugby), David Ribbans), Mako Vunipolasa (Saracens), Mako Vunipola) and Billy Vunipolas), Mako Vunipolas), Mako Vunipolas), Jack Willis (Saracens), Jack Willis
Backs: Joe Cokanasiga (Bath Rugby), Owen Farrell (Saracens), George Furbank (Northampton Saints), Will Joseph (London Irish), Max Malins (Saracens), Jonny May (Gloucester Rugby), Cadan Murley (Harlequins), Jack Nowell (Exeter Chiefs), Guy Porter (Leicester Tigers), Raffi Quirke (Sale Sharks), Henry Slade (Exeter Chiefs), Marcus Smith (Harlequins), Freddie Steward (Leicester Tigers), Manu Tuilagi (Sale Sharks), Jack van Poortvliet (Leicester Tigers), Ben Youngs (Leicester Tigers).