Huw Jones and Adam Hastings will not be able to travel to South America this summer due to injury.
Gloucester fly-half Hastings is suffering from a hamstring injury and Harlequins’ back injury.
Jonny Matthews, an uncapped Glasgow hooker, has been called up to the forward pack.
Finn Russell and Captain Stuart Hogg were not included in the squad after being rested by head Coach Gregor Townsend.
Townsend’s men will face Argentina in three Test matches on 2-9 and 16 July, after Chile’s 25 June clash with Scotland.
Scotland squad
Forwards: Ewan Ashman, Jamie Bhatti (Glasgow), Magnus Bradbury(Edinburgh), Dave Cherry(Edinburgh), Andy Christie/Saracens), Luke Crosbie/Edinburgh), Luke Crosbie/Edinburgh), Scott Cummings/Glasgow), Rory Darge/Glasgow), Matt Fagerson/Glasgow), Zander Fagerson/Glasgow), Grant Gilchrist/Edinburgh), Hamish Watson (Edinburgh), Glen YoungEdinburgh), Glen Young (Edinburgh), Glen Young) (Edinburgh), Glen Young (“Edinburgh”), Glen Young (Edinburgh), Glen Young” (Edinburgh), Glen Young (“Edinburgh), Glen Young” (Edinburgh), Glen Young “Edinburgh”), Glen Young (Edinburgh), Glen Young (“Edinburgh”), Jonny Matthews” (Glasgow), Jonny Matthews), Jonny Matthews”)
Backs: Darcy Graham, Mark Bennett, Matt Currie (all Edinburgh), George Horne, Damian Hoyland, Rory Hutchinson, Sam Johnson (Glasgow), Rory Hutchinson, Sam Johnson (Glasgow), Blair Kinghorn, (Edinburgh), Rufus McLean (both Glasgow), Ali Price (both Glasgow), Kyle Rowe, London Irish), Ollie Smith and Ross Thompson, Sione Tiipulotu, Duhan van der Merwe, Ben White (Worcester), Ben White, Ben White (London Irish), Ben White, Ben White, Ben White, Ben White, Ben White, Ben White, Ben White, Ben White, Ben White, Ben White, Ben White (London Irish), Ben White, Ben White, Ben White, Ben White, Ben White, Ben White, Ben White (London Irish).